50.9 Folate:Active B12

50.9.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 81.653013 1 <0.0001 ***
folate_Active.B12 2.405026 1 0.1 ns
Study 5.359313 1 0.02 *
GA_samp 60.136224 1 <0.0001 ***
folate_Active.B12:Study 7.831834 1 0.005 **
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 6.7473777 1 0.009 **
folate_Active.B12 0.4492223 1 0.5 ns
Study 16.2494864 1 <0.0001 ***
GA_samp 843.1941108 1 <0.0001 ***
folate_Active.B12:Study 4.5843424 1 0.03 *
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 198.6866206 1 <0.0001 ***
folate_Active.B12 1.0610568 1 0.3 ns
Study 0.0121323 1 0.9 ns
GA_samp 468.3134903 1 <0.0001 ***
folate_Active.B12:Study 2.2694976 1 0.1 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 1.589 1.589 0.2 ns
3 Study 1 Inf 8.527 8.527 0.003 **
4 GA_samp 1 Inf 0.097 0.097 0.8 ns
2 folate_Active.B12:Study 1 Inf 1.432 1.432 0.2 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 1.851 1.851 0.2 ns
3 Study 1 Inf 14.060 14.060 0.0002 ***
4 GA_samp 1 Inf 0.177 0.177 0.7 ns
2 folate_Active.B12:Study 1 Inf 1.383 1.383 0.2 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 4.808 4.808 0.03 *
3 Study 1 Inf 41.897 41.897 <0.0001 ***
4 GA_samp 1 Inf 1.163 1.163 0.3 ns
2 folate_Active.B12:Study 1 Inf 0.226 0.226 0.6 ns

50.9.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 15.95 (8.75, 29.04) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.12
StudySTOP 0.77 (0.62, 0.96) 0.02
GA_samp 1.16 (1.12, 1.20) <0.0001
folate_Active.B12:StudySTOP 1.00 (1.00, 1.00) 0.005
hPL (μg/ml)
Var Effect P
(Intercept) 1.54 (1.11, 2.14) 0.010
folate_Active.B12 1.00 (1.00, 1.00) 0.50
StudySTOP 1.28 (1.13, 1.44) <0.0001
GA_samp 1.36 (1.33, 1.38) <0.0001
folate_Active.B12:StudySTOP 1.00 (1.00, 1.00) 0.03
GH2 (ng/ml)
Var Effect P
(Intercept) 0.05 (0.03, 0.07) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 1.00
StudySTOP 1.06 (0.93, 1.20) 0.39
GA_samp 1.33 (1.30, 1.37) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 16.90 (8.16, 35.02) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.18
StudySTOP 0.71 (0.57, 0.90) 0.004
GA_samp 0.99 (0.95, 1.04) 0.78
HOMA-IR
Var Effect P
(Intercept) 4.29 (1.98, 9.31) 0.0002
folate_Active.B12 1.00 (1.00, 1.00) 0.15
StudySTOP 0.62 (0.48, 0.79) 0.0001
GA_samp 0.99 (0.94, 1.04) 0.69
Glucose (mmol/L)
Var Effect P
(Intercept) 6.04 (5.16, 7.06) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.004
StudySTOP 0.86 (0.83, 0.90) <0.0001
GA_samp 0.99 (0.98, 1.00) 0.28

50.9.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:Active B12 ratio and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 103.52216 9.538256 132.2925 86.27438 124.2181
STOP 95.26028 8.205226 102.5693 80.30054 113.0070
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 90.58383 3.238948 255.1716 84.42475 97.19225
STOP 107.75515 3.443906 164.9170 101.16542 114.77411
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.292618 0.0973988 309.6921 2.108763 2.492503
STOP 2.425090 0.0890729 203.7927 2.255676 2.607228
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.69993 1.1456949 Inf 12.617509 17.12604
STOP 10.50458 0.6474221 Inf 9.309302 11.85333
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.557729 0.2993955 Inf 3.016764 4.195701
STOP 2.192979 0.1431559 Inf 1.929606 2.492300
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.531632 0.0815836 Inf 5.374020 5.693867
STOP 4.765678 0.0585860 Inf 4.652224 4.881899

50.9.4 Comparisons

Effect for every 500 unit increase in Folate:Active B12 ratio.
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) folate_Active.B121144.86 / folate_Active.B12644.86 SCOPE 0.96 (0.92, 1.01) 0.1
PRL (ng/ml) folate_Active.B121144.86 / folate_Active.B12644.86 STOP 1.06 (1.01, 1.12) 0.02
hPL (μg/ml) folate_Active.B121144.86 / folate_Active.B12644.86 SCOPE 1.01 (0.98, 1.04) 0.5
hPL (μg/ml) folate_Active.B121144.86 / folate_Active.B12644.86 STOP 0.97 (0.94, 1.00) 0.02
GH2 (ng/ml) folate_Active.B121144.86 / folate_Active.B12644.86 SCOPE 1.00 (0.98, 1.02) 1.0
GH2 (ng/ml) folate_Active.B121144.86 / folate_Active.B12644.86 STOP 1.00 (0.98, 1.02) 1.0
Insulin (uU/ml) folate_Active.B121144.86 / folate_Active.B12644.86 SCOPE 0.97 (0.93, 1.01) 0.2
Insulin (uU/ml) folate_Active.B121144.86 / folate_Active.B12644.86 STOP 0.97 (0.93, 1.01) 0.2
HOMA-IR folate_Active.B121144.86 / folate_Active.B12644.86 SCOPE 0.97 (0.93, 1.01) 0.1
HOMA-IR folate_Active.B121144.86 / folate_Active.B12644.86 STOP 0.97 (0.93, 1.01) 0.1
Glucose (mmol/L) folate_Active.B121144.86 / folate_Active.B12644.86 SCOPE 0.99 (0.99, 1.00) 0.004
Glucose (mmol/L) folate_Active.B121144.86 / folate_Active.B12644.86 STOP 0.99 (0.99, 1.00) 0.004

50.9.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

50.9.6 Plots